User Benefits
Established an effective, fast and simple sample preparation method for analysis of nine macrolide antibiotics in grass carp.
Realized simultaneous quantitative analysis of nine macrolide antibiotics.
The method has a high recovery and good reproducibility.
Introduction
Macrolide antibiotics (MALs) are a class of antibiotics produced by Actinobacteria or Micromonospora. MALs has become one of the fastest growing antibiotics in terms of demand and sales worldwide. Due to its broad-spectrum antibacterial properties, MALS can resist Gram positive bacteria, Mycoplasma, and some Gram negative bacteria. Therefore, it is widely used in the treatment of respiratory and infectious diseases in pigs, cattle, sheep, shrimp, and poultry, or as a feed additive to promote animal growth and development at low doses. Residues of macrolide drugs can cause allergic reactions and lead to the spread of strains carrying resistance factors. Like other veterinary drugs, the monitoring and control of residues of macrolide drugs in animal derived foods has been highly valued by many countries. In this application, we present a complete workflow, from sample preparation using SHIMSEN Styra Al-N, to sample analysis using Shim-pack Scepter C18-120 column on Shimadzu LCMS-8050.